Sunday, 3 July 2011
Rotifers through the light microscope (DIC)
I seldom show any "regular" microscopy pictures but for rotifers I can make an exception. The creature on top is a stack of 20 pics and in my next post I'll show fluorescence of these rotifers.
Unfortunately flickr, picassa and other services do not allow uploading pictures exceeding 1024 pixels in one dimension, I am very sorry but you can't see the critter in full glory (which is almost 10000 px height)
DIC contrast method gives great pictures but the depth of field is so low, that the tiniest movement of the focus screw changes the picture a lot, making things unrecognizable.
Just to give an idea of the resolution, here's a 95% crop of the first image, the area is in the... head of the rotifer, top right. Overall, enough to print a poster-size picture. Also notice that the first picture has different white balance, technically under such magnifications colors don't matter and the camera increased colour temperature comparing to what I saw in the microscope.
Unlike others, this one was very flat and so easy to assemble. And the fluorescent picture is very different which I will show in the next post.
And the last one:
Again, as the depth of field doesn't allow to see the whole object it's possible to assemble pictures in various ways showing different things.
The pictures on top is a real stack of 10 images, while the bottom one shows internal parts that are situated on various focus plans. Basically, it's like taking off a lid out of a toy, just... not literally, microscope is great as it allows seeing what's inside without cutting and damaging things.
Background is modified in all pictures, otherwise it's impossible to make stacks.