More fluorescent rotifers - animations

  Rotifers are very spectacular and interesting to look at through the microscope, however making fluorescent/light microscopy match animations is almost impossible. Even if the animals don't move, there's always something moving inside or just tiny body movements, if you take 2 pictures with 1 second interval, it's already enough not to match the pictures.

  Traffic warning! Animations are roughly 2 mb + pictures, slow connection might cause failure to load the page. Reload the page if pictures do not load. Also notice that Internet Explorer might be very slow here, use another browser if it happens.

 Technically it's impossible to stop living things from moving, the moment they die, they start degradating and losing shape and unless they are plants or resting stages they will not stop moving (at least with some body parts) till death. Fixation is normally used in biology but any kind of solution that does it makes animals lose a lot of structures, shrink and look unnatural.

  Fluorescent photography requires long exposure times making some tasks impossible. Despite all my attempts to make large transition animations I couldn't make 2 matching pictures of entire objects.

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If you don't see the animation immediately, have patience, it's 1,5 mb in size and takes time to load.

  It's the same rotifer from the previous post:

  And how it looks like under different magnifications with UV on:

  The bottom pictures is a stack taken under 100x lens and some time after the first shot, you can also see how coloration changes and that 100x despite being the highest magnification lens for a light microscope is not very good at fluorescence.   And the last one with an animation

The only successful match animation

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